HP-1100 method timetable for for performing trap/wash/elute

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HP-1100 method timetable for for performing trap/wash/elute

Author:		Mark Hail
Posted:		11/28/00; 12:15:56 PM
Topic:		HP-1100 method timetable for for performing trap/wash/elute
Msg #:		28 (top msg in thread)
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Below is a sample method timetable for the HP-1100 operated in trap/wash/elute mode. It has been designed to be used with the associated injection program and valve plumbing. The trap (column 1) and analytical column (column 2) are in-line while the system injects "protein Drano". The analytical column is off-line and the trap is in-line while sample is injected/pre-concentrated on the trapping column and salts are diverted to waste. The contact closure at 4.9 minutes starts the analysis on the MS system. The analytical column is switched in-line, and sample is eluted from the trap and column with a step to 70% B. At 13.9 minutes, the analysis is stopped when the contact opens again. The remaining part of the program re-equilibrates the system back to initial conditions.

Time	Module	Setting	Value
0.00	Binary Pump	flow	0.2 mL/min
0.00	Binary Pump	%B	5%
0.00	Autosampler	External Contact 1	open
0.00	Column compartment	column switching valve	column 2
1.50	Column compartment	column switching valve	column 1
4.90	Autosampler	External Contact 1	closed
4.90	Column compartment	column switching valve	column 2
5.00	Binary Pump	%B	5%
5.10	Binary Pump	%B	70%
13.90	Autosampler	External Contact 1	open
13.90	Binary Pump	flow	0.2 mL/min
14.00	Binary Pump	flow	0.4 mL/min
14.00	Binary Pump	%B	5%
15.90	Binary Pump	flow	0.4 mL/min
16.00	Binary Pump	flow	0.2 mL/min